FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_72-LBD37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_72-LBD37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864461
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG56190.14540190727762553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC38650.066.075266
GGTATAG65550.065.964655
CGTATAC26650.065.8979645
CGTATAG37900.065.35215
GCTATAG39300.065.071435
GTATAGG72000.065.059926
CCTATAC26450.064.9413455
GTATACC23300.064.8624346
GTATACG35000.064.7697756
CGTATAA59650.064.689025
CTATAGC29700.064.5496
CTATACC23600.064.482636
TTATAGC51500.064.397146
GGTATAC37150.064.317235
GGTATAA73000.064.168695
AGTATAG57450.064.121525
GCTATAA62800.063.50545
GCTATAC31300.063.3729865
TATAGCG43250.063.3337977
TTATACG43100.063.149196
Produced by FastQC (version 0.11.5)