FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_71-E2F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_71-E2F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4943585
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG86910.17580359192772047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC35650.066.4367145
CGTATAG46100.066.326875
GGTATAG101850.065.675875
GTATAGC51850.065.651266
GTATAGG106900.065.027826
GCTATAG57450.064.3062065
CCTATAC29850.064.226645
TATAGCG49850.064.214137
AGTATAG71100.064.163345
CTATACC29450.063.7922556
GTATACG47200.063.6693086
TATACGG56150.063.6136867
GGTATAC49100.063.556855
CGCTATA39850.063.38524
CTATAGC37500.063.345756
CGTATAA77350.063.275625
TTATAGC63600.063.038596
TATACCG36000.062.9719477
GCTATAA77350.062.77815
GGTATAA97200.062.6270565
Produced by FastQC (version 0.11.5)