FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_70-BZR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_70-BZR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096238
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG74990.1830704172950888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG38600.065.8884355
GGTATAG77800.065.8749165
GTATAGG82000.065.3165746
CGTATAC27750.065.302355
CCTATAC24650.064.9995965
GTATAGC40000.064.9816746
GCTATAG44150.064.499415
TTATAGC52350.063.8856436
GGTATAC41100.063.8383265
CGTATAA60500.063.837495
GGTATAA76900.063.825385
GTATACG40350.063.8111156
TATACGG45300.063.709947
TATAGCG41650.063.413757
CTATACC26450.063.089166
CTATACG27200.063.0215766
AGTATAG59850.062.952595
GCTATAA62200.062.5426835
TATAGCC28550.062.4907267
CGGTATA53700.062.475024
Produced by FastQC (version 0.11.5)