FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_7-WRKY60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_7-WRKY60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326808
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG75720.22760556064551968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG72300.066.477875
CGTATAG34850.065.946415
GTATAGG76550.065.346076
CGTATAC22750.065.194825
GTATAGC34750.065.0288856
CCTATAC19300.063.9802635
TATAGCG37400.063.5993777
TATACGG40450.063.560077
CTATACC23900.063.375096
GCTATAG44550.063.0516475
CGCTATA27850.062.801994
TATAGGC42850.062.6939967
GTATACG36950.062.67186
CGGTATA45300.062.3938454
GATATAG56800.062.2016145
GGTATAA66500.062.123645
CTATAGC26100.062.05396
CGTATAA47150.061.874685
CCTATAA31250.061.677995
GGGTATA65700.061.65554
Produced by FastQC (version 0.11.5)