FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_69-NAC080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_69-NAC080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15353486
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG352650.22968725148152022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC112050.066.470135
CGTATAG150900.066.188065
GTATAGG294100.065.518166
GGTATAG273850.065.2147455
GTATAGC159150.064.9551546
CCTATAC95950.064.753135
CTATACC99300.064.7176366
CGTATAA237450.064.132665
GGTATAA284300.063.703615
TATAGCG171200.063.6521077
GCTATAG175250.063.5391435
AGTATAG221400.063.330495
TATACGG186900.063.2840657
TTATAGC202450.062.96856
GTATACG152900.062.851256
GGTATAC143750.062.5443425
CTATAGC124600.062.4704636
CGCTATA134650.062.120654
TATACCG116350.062.058587
CGGTATA197800.061.9550324
Produced by FastQC (version 0.11.5)