FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_68-MYB30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_68-MYB30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9137068
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG243690.2667048116529285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG92500.066.296375
GTATAGG177100.065.2043466
GGTATAG156300.065.130385
CGTATAC65950.065.084765
GTATAGC87400.065.041646
CCTATAC59100.064.933495
CTATACC63300.064.4383546
GGTATAA168850.063.9151345
TATACGG102700.063.6280867
CGTATAA142000.063.5842065
AGTATAG127850.063.370545
GTATACG84850.063.3682376
GGTATAC83250.063.2414555
GCTATAG103500.063.1035165
CGCTATA80400.062.872594
TATAGGC107450.062.54087
GCTATAA136900.062.5288055
CGGTATA116900.062.124434
TATAGCG80850.062.002427
TTATAGC115550.061.9117286
Produced by FastQC (version 0.11.5)