FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_67-HRS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_67-HRS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577886
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG77570.21680400102183245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG37200.066.490865
GGTATAG67600.065.9338155
GTATAGG72400.065.669916
CCTATAC24550.064.9828645
CGTATAC25400.064.87435
GGTATAA67150.064.552165
GTATAGC34800.064.340776
TATACGG40000.064.285477
CTATACC23950.063.981456
GGTATAC34550.063.894995
TATAGCG32500.063.5117577
GTATACG32700.063.3372846
GCTATAA54350.063.0829545
TATAACG27350.063.0630657
GTATACC21150.063.0231486
CGTATAA56850.062.955065
TATACCG25800.062.919287
TTATAGC46900.062.8094186
CTATAGC28600.062.7533386
AGTATAG52900.062.629625
Produced by FastQC (version 0.11.5)