FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_66-ARID6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_66-ARID6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5889209
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG157410.267285470765259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC43500.068.034115
CGTATAG56000.066.840755
CTATACC42600.066.43316
CCTATAC41350.065.311165
GTATAGG100350.064.9791646
GGTATAG96800.064.904765
GGTATAA111000.064.291395
GTATAGC57250.064.159266
CTATAGC47250.063.9672286
TTATAGC71150.063.6708266
GGTATAC53200.063.191315
GCTATAG68150.063.1884655
TATAGCG54550.062.9736187
GTATACG53650.062.9220966
CCTATAA72200.062.8902135
CGTATAA97750.062.770915
GCTATAA93600.062.5641025
TATACGG61900.062.5037967
CGCTATA57200.062.441794
AGTATAG81350.062.18085
Produced by FastQC (version 0.11.5)