FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_65-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_65-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6734868
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG175900.2611780958438978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG63600.066.66655
CGTATAC44800.066.062425
GGTATAG126000.065.7188345
GTATAGC64750.065.482476
GTATAGG136300.065.475196
CCTATAC41150.064.865965
GGTATAA139750.064.359465
CTATACC42800.064.16356
CTATAGC48050.064.069516
GCTATAG79200.064.047915
AGTATAG101100.063.876735
CGTATAA104000.063.8447235
GGTATAC67450.063.691075
GTATACG65200.063.6354686
TATAGCG63350.063.394417
CGGTATA93900.063.1862224
CGCTATA59550.063.034764
TATACGG72250.062.9934167
TATAAGG113650.062.793757
TTATAGC80900.062.7452776
Produced by FastQC (version 0.11.5)