FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_64-NAC080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_64-NAC080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6532080
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG190060.290963980845305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC48950.066.46685
CTATACC43400.066.1801766
CGTATAG64100.066.03925
GTATAGC61450.065.9266746
GTATAGG113050.065.543536
GGTATAG97300.065.366555
CCTATAC44300.064.8356555
CGTATAA107950.064.718715
TATACGG78350.064.073847
GTATACG62450.063.8626446
TATAGCC49100.063.7689677
GGTATAA124600.063.6513985
GCTATAG69500.063.5759745
TATACCG51450.063.508137
CTATAGC55150.063.4984366
GCTATAA109150.063.334115
CTATACG52350.063.286026
TATAGCG62750.063.2218447
TTATAGC83650.063.193586
AGTATAG89650.063.1397255
Produced by FastQC (version 0.11.5)