FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_63-MYB30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_63-MYB30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7707873
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG277360.35983986762625697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC54550.066.563245
GGTATAG143100.065.684055
CCTATAC45150.065.313315
CGTATAG75150.065.260585
GTATAGG153900.065.143266
CTATACC51300.064.847776
GTATAGC74200.064.8232356
GCTATAA124400.063.803725
CGTATAA122300.063.7265785
TATACGG87350.063.674177
GTATACG74950.063.661176
GGTATAC74300.063.653135
GGTATAA148850.063.616735
AGTATAG110300.063.5556375
TTATAGC96400.063.2125056
CGGTATA103450.063.199074
TATAGCG71650.063.1752747
CGCTATA66950.063.012774
TATACCG56350.062.5121777
GCTATAG88050.062.334075
Produced by FastQC (version 0.11.5)