FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_62-HRS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_62-HRS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215248
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG120950.23191610446904923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG53400.065.836885
GTATAGC52200.065.339916
GGTATAG95050.065.142685
GTATAGG99950.065.134046
CGTATAC40450.065.034495
CCTATAC35900.064.9943855
TATACGG59050.064.454537
CGTATAA84700.064.099235
TATAGCG51050.064.0023047
GTATACG50350.063.849946
GTATACC33550.063.603616
CTATACC37150.063.5608066
GGTATAC51050.063.5226255
GGTATAA97750.063.343345
TTATAGC70250.063.341056
TATACCG40100.063.246737
AGTATAG72900.063.0539475
GCTATAG63800.062.4521645
GCTATAA87500.062.407865
CGCTATA50750.062.3816684
Produced by FastQC (version 0.11.5)