FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_61-ARID6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_61-ARID6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805347
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG107130.18453677273727134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC42250.066.319245
CCTATAC39000.065.029015
GTATAGC60000.064.656226
CGTATAG62850.064.6190645
CTATACC37900.064.4237756
CTATAGC46900.063.7710046
TATAGCG57000.063.271787
CGTATAA98650.062.7638975
TTATAGC75050.062.4108166
AGTATAG83600.062.3048865
GCTATAG69900.062.2553865
GCTATAA93350.061.905535
TATACGG69100.061.8107267
TATAGCC42100.061.4859477
TATAGCA75600.061.1696367
CGCTATA54300.061.1364334
TATAGGT114400.060.8795557
TATACCG42350.060.7099887
CCTATAA70750.060.617465
GATATAG83850.060.450385
Produced by FastQC (version 0.11.5)