FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_60-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_60-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6701434
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG188540.2813427693237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC48500.066.932675
GGTATAG119150.065.9398045
CGTATAG65950.065.824715
CCTATAC43550.065.463835
CTATACC43350.065.362386
GTATAGC62650.064.9925846
GTATAGG133750.064.966546
TATACGG69300.064.307517
GCTATAG78900.063.799275
CGTATAA105500.063.5625885
GGTATAA131000.063.5532265
TTATAGC80750.063.204026
AGTATAG96250.062.838565
CGGTATA83650.062.5183074
GGTATAC62450.062.4560285
CTATACG46850.062.19676
TATAGCG62300.062.156287
GGGTATA115700.062.010364
CGCTATA57550.061.99934
CTATAGC47650.061.960016
Produced by FastQC (version 0.11.5)