FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_6-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_6-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081559
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG33650.16165768061342484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG23050.066.023377
GGTATAG42650.065.047935
CGTATAG24700.064.728965
GTATAGG43150.064.294196
GTATAGC20850.064.096846
TATAGCG22950.063.2622647
GTATACG20800.063.2417456
TTATAGC27200.062.7668386
CGTATAC16000.062.753975
CGCTATA19000.062.4202424
CGTATAA30850.062.2581945
CGGTATA27450.062.195194
GCTATAA30500.061.825595
GCTATAC17600.061.6209455
GGGTATA39250.061.5910644
CCTATAC14050.061.503525
CTATAGC18800.061.2233856
GGTATAA40250.061.016965
TATAAGG37050.060.9047057
GGCTATA30350.060.8631744
Produced by FastQC (version 0.11.5)