FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_59-NAC080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_59-NAC080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960796
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG164260.23597875875115432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC49500.067.423845
CGTATAG67400.066.2828755
GGTATAG115600.065.973425
GTATAGC67450.065.818816
CCTATAC47600.065.631885
GTATAGG123400.065.630586
CTATACC49050.065.5461046
CGTATAA114000.064.536415
GTATACG62900.064.2950446
CTATAGC55200.064.010716
GCTATAG74000.063.916845
TATACGG75500.063.758137
GGTATAA129400.063.722925
TTATAGC88750.063.464066
AGTATAG92300.063.44895
TATAGCG63800.063.1678247
CCTATAA89650.063.1001055
GTATACC43750.062.771426
GGTATAC66300.062.7391555
CGGTATA87300.062.714864
Produced by FastQC (version 0.11.5)