FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_58-MYB30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_58-MYB30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5373400
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG179010.33314102802694756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC51150.066.48446
CGTATAC38500.066.337825
CGTATAG49000.066.1171345
GGTATAG83450.066.031825
CCTATAC35600.065.1572345
CTATACC37450.065.114856
GGTATAC47250.064.937695
GTATACG44200.064.906876
TATACGG53600.064.685057
CGTATAA88800.064.653895
GTATAGG92150.064.543526
CTATAGC41650.064.0927056
AGTATAG70450.063.8644335
GGTATAA100950.063.7691465
TTATAGC64550.063.577126
TATAGCG45500.063.4362647
GCTATAG54200.063.259525
GCTATAA84650.063.1118855
CTATACG39750.063.107526
GTATACC33450.063.069636
Produced by FastQC (version 0.11.5)