FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_57-HRS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_57-HRS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849844
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG142150.3692357404611719No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC27850.067.203435
CTATACC27900.066.5814366
GGTATAG65850.066.407495
GGTATAC33250.066.284465
CGTATAG40950.066.208035
GTATAGC39950.066.20156
GGTATAA70250.065.783855
CCTATAC25450.065.568225
CGTATAA66450.065.544645
GTATACG35100.065.182866
GTATAGG70500.064.955126
GTATACC22900.064.620126
TATACGG41500.064.318167
TTATAGC44500.064.306696
GCTATAG45150.064.233215
AGTATAG49200.063.9230425
TATAGCG36650.063.6662187
CTATACG29300.063.6388636
CCTATAA46600.063.4356845
TATACCG30050.062.981067
Produced by FastQC (version 0.11.5)