FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_56-ARID6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_56-ARID6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027038
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG88300.2192678589077133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG42050.066.479565
CGTATAC31500.065.975585
GGTATAG72000.065.697915
GTATAGG79400.065.2592246
CCTATAC27700.065.174435
GTATAGC40500.064.877226
CTATACC26700.064.470526
TATAGCG36900.064.190337
GTATACG39500.063.862446
TATACGG45300.063.7953727
GGTATAA72900.063.734985
CGTATAA66100.063.5165755
GGTATAC38850.063.1297845
CTATAGC31350.063.0548066
CGGTATA52000.062.3711434
CGCTATA38750.062.209234
GCTATAG49150.061.8590935
GCTATAA63600.061.8324625
TTATAGC53050.061.7302136
AGTATAG63050.061.6504555
Produced by FastQC (version 0.11.5)