FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_55-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_55-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287928
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG43500.19012836068267883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC18300.066.527455
CGTATAG22100.065.852735
GGTATAG38050.065.371385
CCTATAC19050.065.193775
GTATAGG40350.064.5936
GTATAGC22850.064.456796
CTATACG19250.063.789496
AGTATAG31350.063.496165
CTATACC18350.063.486436
GTATACG22900.063.2466666
CGTATAA42000.063.221565
GGTATAC23300.063.211915
TATACGG27200.063.150367
TTATAGC30200.062.9021766
GGTATAA44200.062.6867375
CGCTATA23500.062.6739354
CTATAGC18600.062.445036
TATAGCG23600.062.4077
GCTATAC22450.062.176935
CCGTATA20650.062.1754264
Produced by FastQC (version 0.11.5)