FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_54-HHO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_54-HHO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233864
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG36150.065.9020845
GTATAGG64200.065.3347856
CGTATAC26350.064.92155
GGTATAG59100.064.1063845
CTATACC27200.064.050246
GTATAGC36050.063.8529476
TTATAGC44150.063.3105936
CTATAGC26750.063.035266
GGTATAC35850.062.94065
GTATACG34250.062.6123776
TATAGCG34500.062.5633057
GTTATAC39500.062.438485
GCTATAC31450.062.402585
TTATAGG75750.062.2540446
CCTATAC24750.062.051125
GATATAG49400.061.751835
TATAGCA45800.061.7162447
TATACGG41100.061.709187
CGGTATA42900.061.6488274
GGTATAA62000.061.615695
Produced by FastQC (version 0.11.5)