FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_52-DOF2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_52-DOF2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7595804
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAA449400.065.575026
CTATAGC71450.065.314076
TTATAGC87800.064.9849246
GTATAGG108500.064.935616
CGTATAC79450.064.8577655
CTATACC66450.064.700876
TTATACC78000.064.5385746
GGTATAA155650.064.256675
GTATAAA400900.064.2498256
CCTATAA150950.064.19485
TTATAGG119250.064.156936
GCTATAA166150.064.070015
CTATACA137200.064.024446
CGTATAA163550.063.997685
TCTATAA206000.063.9537855
GATATAC79550.063.9406975
GTATACG72050.063.8962066
CATATAC101600.063.8709535
TCTATAG86250.063.840875
TCTATAC99550.063.780595
Produced by FastQC (version 0.11.5)