FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_51-AT5G46915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_51-AT5G46915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761320
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG98150.066.3639146
CGTATAC42200.065.90185
CGTATAG49100.065.617665
GGTATAG87250.065.112335
TTATAGC61500.064.730626
CCTATAC37350.064.0631265
GTATACG50100.064.028636
GTATAGC50550.063.9430586
TATAGCG54650.063.8813177
TATACGG65450.063.869247
CTATAGC42350.063.768546
GCTATAC45000.063.5892075
TATAGCC39300.063.5534557
GCTATAA81600.063.275975
CTATACG38450.063.231156
GGTATAC50850.063.0154575
CTATACC38550.062.9763836
CGCTATA50300.062.9394844
GCTATAG57300.062.8207865
GGTATAA90600.062.8205835
Produced by FastQC (version 0.11.5)