FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_50-HHO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_50-HHO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14674109
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG161200.065.7565845
CCTATAC112750.065.560855
GGTATAG276350.065.3841555
GTATAGG304900.065.3428346
CGTATAC125800.064.9055565
CTATAGC127900.064.5236746
TATAGCG158250.064.483487
TTATAGC190500.064.035186
TATACGG186850.063.843557
CTATACC117300.063.7037356
GTATAGC157450.063.567756
GCTATAC146050.063.2599035
GTATACG159150.063.108556
GATATAG219650.062.7837225
GCTATAG189150.062.753685
CGCTATA149800.062.750554
CTATACG129600.062.7325336
GGTATAC160350.062.265395
CGGTATA198150.062.0749634
TATAGGC190750.061.8963437
Produced by FastQC (version 0.11.5)