FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_5-GAMMAH2AX.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_5-GAMMAH2AX.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4841511
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG103630.21404474760049083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG93100.065.568975
CTATAGG52750.065.057786
GTATAGG100300.064.9428566
CGTATAG50850.064.736815
TATAGCG47750.063.737367
CCTATAC32100.063.4264765
CGTATAC35150.063.2971845
CCTATAG35900.063.0467155
GTATAGC48600.062.983166
CGGTATA63800.062.8371624
TATACGG55200.062.8033377
TTATAGC61800.062.77636
GCTATAG60400.062.6675835
CGCTATA42350.062.5309564
CGTATAA70900.062.218785
GTTATAC59300.061.6473275
GGTATAA94250.061.5397575
GATATAG78200.061.5104065
GGGTATA89200.061.4941524
GTATACG48150.061.1742176
Produced by FastQC (version 0.11.5)