FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_48-DOF2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_48-DOF2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5776974
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC56500.066.126685
CGTATAG64600.065.633275
CCTATAC48650.065.291555
GTATAGC59000.065.222086
GTATAGG102800.064.792956
GTATACG60750.064.782876
GGTATAG97600.064.589055
TTATAGC73400.064.532216
TATACGG71250.064.171317
CCTATAA89050.064.112135
GGTATAC58350.064.030125
CTATACC52250.064.006726
CTATACG49150.063.986766
GCTATAC61650.063.9506265
TTATACC55200.063.9449236
GCTATAA107000.063.8516925
CTATAAA237500.063.661246
CTATAGC56150.063.5483636
GGTATAA112150.063.4773985
CGTATAA108650.063.2683835
Produced by FastQC (version 0.11.5)