FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_47-AT5G46915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_47-AT5G46915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8314440
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG91700.065.886475
CGTATAC79250.065.112745
GTATAGG158900.065.102956
CTATACC70350.064.249786
CCTATAC67650.064.228395
GGTATAG140950.063.937265
CTATAGC70600.063.0312086
TTATAGC105500.062.971756
TATACGG105100.062.9106987
CGTATAA140950.062.721065
TATAGCC66250.062.6801837
GCTATAC82450.062.6704865
GTATAGC89600.062.66726
TATAGGC96150.062.6540687
GTTATAC99850.062.5407945
CCTATAA116500.062.4312175
GCTATAA141300.062.367635
GGTATAC86050.062.365985
GTATACG89400.062.3378036
TTATACC81850.061.9331176
Produced by FastQC (version 0.11.5)