FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_45-WIP5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_45-WIP5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314987
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG45250.066.250545
GTATAGG87900.065.065546
GGTATAG78250.064.9990845
CGTATAC36850.064.550355
CTATACC34950.064.3555456
GTATAGC47500.064.216266
CCTATAC31800.063.9110875
TTATAGC57150.063.8396156
TATAGCG47400.063.833687
CGCTATA46700.063.5193674
GGTATAC44600.063.451355
CCTATAA54700.062.9906545
GGGTATA73350.062.855394
CTATAGC36250.062.8195956
GTATACG46400.062.798496
CGGTATA58750.062.637594
TATACGG52500.062.1633537
TATAGCC32450.062.0897457
TATAGGT86950.061.993367
TATACCC26150.061.93297
Produced by FastQC (version 0.11.5)