FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_44-DOF2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_44-DOF2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4710138
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG54350.065.458675
GTATAGC55100.065.329536
CGTATAC39900.065.229775
GGTATAG96250.064.367055
GTATAGG107600.064.339746
CCTATAC35000.064.167155
CTATACC34950.063.5583046
CGGTATA64050.063.300934
TATACGG58350.063.248877
TATAGCG48750.063.146497
TTATAGC63300.062.7246066
GTATACG51450.062.485026
GGTATAC49200.062.356295
GCTATAC42450.061.9706275
TATAGGC59200.061.6907357
GTTATAC55950.061.648515
CTATAGC42000.061.6351136
TATAGGT105400.061.5665057
CGCTATA44000.061.4571724
TTATAGG117600.061.3376436
Produced by FastQC (version 0.11.5)