FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_43-AT5G46915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_43-AT5G46915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922804
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG62800.065.894896
GGTATAG64300.064.792895
CGTATAC24250.064.768115
CCTATAC20350.064.2885745
TATAGCG35000.063.8644877
CGTATAG31550.063.8630685
GTATAGC35500.063.6547436
CTATACC23200.063.477646
CTATAGC26850.063.1864666
GTATACG33850.063.0373276
GCTATAC27350.062.9266245
TATAGCC24300.062.9074067
TTATAGC40250.062.8346256
GGTATAC33200.062.796415
TATACGG40150.062.729757
CGGTATA38150.062.442354
CTATACG24650.062.439936
GCTATAG39500.062.345085
TATACTG44450.061.6979757
CGCTATA29000.061.035034
Produced by FastQC (version 0.11.5)