FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_42-TLP9-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_42-TLP9-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4231742
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46600.11012013492315931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG80800.066.065286
CCTATAC33950.066.046285
GGTATAG74850.065.895755
CGTATAC41550.065.415415
GTATAGC45950.065.241726
TTATAGC54350.065.198866
CGTATAG45300.065.0195545
CTATAGC38750.064.7258156
CGTATAA77750.064.562695
TTATACC39450.064.5527046
GCTATAG48150.064.512935
TATAGCG44300.064.355257
CTATACC34550.064.089336
GGTATAC43800.063.971795
CCTATAA62050.063.9295125
GCTATAA79500.063.8015145
GCTATAC45200.063.692975
AGTATAG69450.063.3634035
TATAGCA69100.063.329987
CTATAAA170550.063.3162236
Produced by FastQC (version 0.11.5)