FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_40-NAC096-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_40-NAC096-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784409
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG41830.11053245037732444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC33450.066.097385
CGTATAG40600.066.003365
TTATAGC46750.066.000976
TATAGCG37850.065.992667
GTATAGG67550.065.564936
GTATAGC39800.065.2205056
CCTATAC31850.065.0242845
CTATAGC33550.064.961926
TATACGG46650.064.7176067
GGTATAG62700.064.666485
GGTATAC39450.064.557655
GGTATAA71250.064.369655
CTATACC30850.064.183686
GCTATAA67750.064.1321265
GCTATAG46000.063.882925
GTTATAC44150.063.6279875
AGTATAG57850.063.6173675
CGTATAA65800.063.427545
GCTATAC36200.063.298885
GTATACG36550.063.267026
Produced by FastQC (version 0.11.5)