FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_4-LBD4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_4-LBD4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940647
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG33140.17076779032971992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG43100.065.911886
CGTATAG21350.065.382275
GGTATAG40600.064.97265
TATACGG25750.064.671767
GTATAGC21000.064.639416
TATAGCG22250.063.995497
CTATAGG23300.063.5139926
GCTATAG25800.062.919215
CGGTATA25650.062.7415774
CCTATAC12850.062.619515
GTATACG21000.062.4736566
CTATAGC15600.062.1212466
ATAGGGG13350.062.1086468
CGCTATA17250.062.060794
TATAGGC27150.061.852377
CGTATAC13750.061.828485
TTATAGC26500.061.6532486
GGTATAC20500.061.608175
TATAGGT49500.061.5599067
TTATACC18650.061.529026
Produced by FastQC (version 0.11.5)