FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_39-TGA1-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_39-TGA1-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6128765
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC56450.066.3175
GTATAGG118400.066.161826
CGTATAG66750.066.095045
CCTATAC45200.065.406865
GTATAGC64550.065.2582246
CTATACC46650.065.023826
GCTATAC59700.064.9337545
GGTATAG102500.064.75135
CTATAGC52450.064.237096
GTATACG64600.064.016226
GGTATAA119400.063.996085
GGTATAC60400.063.833655
TTATACC57750.063.7336466
GCTATAG74200.063.7025035
TATAGCG65200.063.6417547
TATACGG81050.063.5850147
TTATAGC81750.063.5113266
TATAGGC70950.063.316617
TATAGCA92100.063.2878577
CTATACG49150.063.2824486
Produced by FastQC (version 0.11.5)