FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_38-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_38-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382554
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC20350.066.702625
CGTATAG26750.065.91475
CCTATAC18100.065.910015
GTATAGG45600.065.826086
CTATACC19000.064.4451146
GTATAGC28500.064.4451146
GGTATAG44800.064.034115
AGTATAG36950.063.341345
TTATAGC31050.063.3213656
GGTATAC25950.063.2282375
GCTATAC24350.063.2163285
GTATACG24650.063.156596
GGTATAA45550.062.979765
TATACGG26700.062.8921477
CCTATAA31750.062.8068055
TATAGCG25200.062.748647
TATAGGC29000.062.7294967
GCTATAG30200.062.670895
GCTATAA41100.062.563515
GGGTATA39700.062.3023574
Produced by FastQC (version 0.11.5)