FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_37-TLP9-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_37-TLP9-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939329
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC30500.066.98655
CCTATAC28450.066.771625
CTATACC26650.066.161856
CTATAGC24950.066.042686
CGTATAG30700.065.866395
GTATAGG39400.065.7067266
GTATAGC27950.065.587916
CTCCCTA2350.065.501582
GTATACG28050.065.354096
GGTATAG38800.065.189995
TTATAGC34100.065.146896
GCTATAC32600.065.139655
GATATAC31000.065.116115
CTATAAA160500.065.064436
CGTATAA64100.064.9476245
AGTATAC33650.064.874475
TTATACC27550.064.76246
GGTATAC26850.064.756965
GGTATAA61300.064.718285
GCTATAA60500.064.7066655
Produced by FastQC (version 0.11.5)