FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_35-NAC096-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_35-NAC096-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887752
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG65750.065.710475
CCTATAC43900.065.584125
CGTATAC52200.065.409785
GTATAGG120050.064.896536
GTATAGC66850.064.681566
CTATACC46850.064.665366
GGTATAG107600.064.6024555
GTATACG59900.064.12676
TATACGG66250.064.1045467
CTATAGC51050.063.9365656
GGTATAC62750.063.276955
GCTATAC58450.062.9643365
TATACCG49650.062.6380967
TATAGCG61050.062.6311727
GCTATAG69800.062.5493135
CGCTATA62700.062.5462764
GGTATAA116000.062.2766845
GCTATAA96450.062.2412765
GTATACC44300.062.149046
CTATACG47850.062.1441736
Produced by FastQC (version 0.11.5)