FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_34-TGA1-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_34-TGA1-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339647
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC29000.065.74585
CGTATAG37750.065.7050555
CCTATAC28250.064.890685
GTATAGG62000.064.663976
CTATAGC29000.064.539456
GGTATAC34750.064.430975
GTATAGC35500.064.350856
GTATACG33950.064.0944066
GGTATAG56950.064.009365
TATACGG39400.063.4852457
TTATAGC43150.063.3198286
CTATACC28050.063.2331126
CTATACG28950.063.20086
GCTATAA56250.062.8779145
TATACTG46500.062.7445647
CGTATAA58700.062.6374555
CGCTATA37150.062.4344374
CTATAAA112600.062.29396
TTATAGG73150.062.2681776
GGTATAA66200.062.25255
Produced by FastQC (version 0.11.5)