FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_33-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_33-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473539
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG53540.11968153178054332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG47450.065.983585
CGTATAC36850.065.313065
GGTATAG82250.065.11615
GTATAGG91950.065.018146
CTATAGC37050.064.204426
CCTATAC31400.064.1714555
CTATACC36050.063.9476136
GTATAGC46750.063.9028856
GCTATAG55800.063.50745
CGTATAA73350.063.287785
TATACGG57400.063.260077
TATAGGC53200.063.191117
CGGTATA58500.063.087844
TTATAGC58200.063.0516856
GTATACG44950.062.8819276
GGTATAA86700.062.8229875
GCTATAC45450.062.652675
CGCTATA47950.062.4502564
TATAGCG47000.062.446537
AGTATAG67200.062.4164355
Produced by FastQC (version 0.11.5)