FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_30-NAC096-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_30-NAC096-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7060456
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC58550.065.4230045
GTATAGG149800.064.499316
CGTATAG77600.064.1935655
CTATACC50400.064.064066
TATAGCG74400.063.9910477
CCTATAC51000.063.5161365
GTATACG76700.063.4417276
GGTATAG135750.063.4183585
TTATAGC90650.063.2491046
GTATAGC77350.063.134736
GCTATAC63400.062.9011845
CTATAGC62200.062.877416
CGCTATA72650.062.7892344
GCTATAG93600.062.601145
TATACGG90300.062.563187
GGTATAC78900.062.55955
CTATACG55050.062.211216
GCTATAA114150.061.9346435
AGTATAG110050.061.889815
TATAGCC55400.061.8168567
Produced by FastQC (version 0.11.5)