FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_3-HB23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_3-HB23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766793
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG64160.1703305703286589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG71100.065.488366
CGTATAG38800.064.91675
CTATAGG40200.064.570416
GGTATAG69350.064.164735
CGTATAC28950.063.923835
CCTATAG27500.063.350835
TATACGG47250.063.2266587
TATAGCG38850.062.6703837
GTATAGC39150.062.549236
CTATAGC29050.062.3791246
GGGTATA63900.062.356044
TTATAGC50350.062.323046
CCTATAC23900.062.2080655
CGCTATA33950.062.0315174
GGTATAC39900.061.9872255
CGTATAA53400.061.77695
GCTATAG43450.061.7534565
TATAGCC30100.061.596147
GTATACG39250.061.587716
GCTATAC35100.061.294815
Produced by FastQC (version 0.11.5)