FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_29-TGA1-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_29-TGA1-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4030844
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG43900.066.22335
GTATAGG84800.065.760756
CGTATAC34250.065.576585
CCTATAC27350.065.235985
GGTATAG76750.064.544495
GTATAGC44600.064.399516
GTATACG43650.063.7974176
CTATACC30100.063.6925136
TATAGCG44950.063.585967
TTATAGC53400.063.482916
GGTATAC41000.063.227895
TATACGG50650.062.922777
GCTATAG52400.062.825025
CTATAGC35750.062.433686
CGGTATA52250.062.335824
CGTATAA61250.062.3150525
GCTATAC37750.062.2768445
TATAGGC53200.062.274077
TATAGCC31200.062.2310757
TATAGGT89550.061.84217
Produced by FastQC (version 0.11.5)