FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_28-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_28-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4850601
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG55180.11375909913019025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC50500.066.157856
CGTATAC40000.065.157695
CGTATAG52450.065.0989155
GTATAGG100450.064.9180456
CCTATAC35250.064.707995
TATAGCG52150.064.601327
GGTATAG90300.064.5828555
AGTATAG72450.063.932115
TATACGG61300.063.918567
GTATACG51450.063.780356
CTATACC36800.063.503566
GGTATAC47600.063.2063835
CTATAGC42450.062.880546
CGTATAA80500.062.580075
GGGTATA80400.062.5708774
GGTATAA91000.062.4329765
GCTATAG62000.062.4069375
TTATAGC64950.062.3733146
TTATACC42950.062.0670666
CGCTATA51600.062.0355764
Produced by FastQC (version 0.11.5)