FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_27-SAP11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_27-SAP11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428672
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG48060.10852011618832913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG48050.065.6027455
GTATAGG100400.065.0580756
GGTATAG89650.064.859165
CCTATAC29500.063.9230355
CTATAGC37750.063.391326
TATACGG56350.063.2032787
CGCTATA39950.062.8779344
CGTATAC34350.062.7400135
TTATAGC64050.062.597376
GCTATAC41650.062.327485
GTATAGC49450.062.1889536
GCTATAG61650.062.026565
TATACCG37000.061.744357
GGGTATA84150.061.5731774
TATAGCG51450.061.470797
GGTATAC49900.061.4177935
CGGTATA57450.061.202154
GATATAG76000.061.1789555
CGTATAA63800.061.142735
TATACTG73950.061.1237647
Produced by FastQC (version 0.11.5)