FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_26-MYB34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_26-MYB34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892837
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG50130.1287749782485113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC28550.065.804485
GTATAGC38950.065.749276
GGTATAG75850.065.727425
CGTATAG37500.065.3061455
GTATAGG79050.064.925536
TATAGCG40600.064.369757
TATACGG45200.063.7787557
CTATAGC33200.063.542826
CGGTATA48650.063.3549164
CCTATAC24900.063.226695
ATAGCGC7700.063.155538
CTATACC26500.062.577726
TTATAGC52950.062.2403766
GCTATAG49350.061.8891265
TATACTG61600.061.565287
GGGTATA71000.061.5448044
GTATACG41050.061.533466
TATAGCC31250.061.350467
CGTATAA54350.061.1520925
TATACCG30100.060.9048777
Produced by FastQC (version 0.11.5)