FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_25-TCP23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_25-TCP23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221113
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG74840.1772992099477081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG45000.065.145295
GGTATAG78050.064.945185
GTATAGG80400.064.830856
GTATAGC43850.063.981776
CTATAGG45900.063.7917146
CCTATAC32900.063.5853045
TATACCG41550.063.566377
CCTATAG30650.063.0028345
CGTATAC39450.062.8710485
TTATAGC58750.062.8197336
TATAGCG45750.062.3951077
CTATACC36550.062.308186
CGCTATA37800.062.2837524
CGGTATA57800.062.2181054
GCTATAC38950.061.8818445
CGTATAA63450.061.4193275
TATAGGT100500.061.2977877
TATAGCA48400.061.147197
TATACGG51650.061.0924727
CTATAGC39350.061.0756
Produced by FastQC (version 0.11.5)