FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_24-CRC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_24-CRC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779207
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC13850.065.920945
CGTATAG19150.065.395355
TATACGG23400.065.327827
GGTATAG39800.064.3369755
GTATAGG42000.064.048616
GTATAGC22200.063.8167466
TATAGCG21100.063.4963767
CGGTATA24900.063.3592154
ATACGGG7650.062.645778
CCTATAC14200.062.5717055
CGCTATA15500.062.5144844
GTATACG21400.061.788926
GCTATAC18700.061.7312365
TATACCG15150.061.187947
GGTATAC22200.061.1380165
TATAGGT42750.060.9610947
TATACTG30300.060.9570437
GGGTATA35150.060.8062824
GGTATAA37150.060.6400645
CGTATAA24900.060.5494965
Produced by FastQC (version 0.11.5)