FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_23-SAP11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_23-SAP11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715008
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG29850.066.448385
GGTATAG65750.065.016125
GTATAGG71150.064.850826
CGTATAC20950.064.286885
GTATAGC32550.064.053216
TATAGCG33600.063.717367
TATACGG35700.062.6149837
TATACCG21950.062.154957
TTATAGC40300.062.1517876
GCTATAG39150.061.8329475
GCTATAC23900.061.767535
GTATACG33100.061.7206546
CTATAGC21200.061.548666
ATACGGG9850.061.440618
GGGTATA58900.061.411674
GGTATAC32150.061.3682565
CGCTATA22700.061.334224
TTATACG38850.061.0498126
CTATAGT41900.060.863816
CGGTATA37000.060.7931864
Produced by FastQC (version 0.11.5)