FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_22-MYB34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_22-MYB34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144838
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG2280.15741725237851945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.1532746931054005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCC150.002207414770.1080369
TTCCGGG301.01685146E-770.0110651
GTTCCGG301.01685146E-770.0110650
GGTTCCG301.01685146E-770.0110649
ATAGGTC207.9533456E-569.914378
TACTTGC150.002231776769.914379
CGCGGTT150.002231776769.914371
ACCGCTA150.002231776769.914372
CGGCATA150.002231776769.914372
TAGCGGA252.8491013E-669.914379
GCGCCAT150.002231776769.914371
TAACGTG150.002231776769.914379
CCGCTTA150.002231776769.914372
ATACTGG500.069.914378
AGTATAG1350.069.914375
TTATAGC1650.069.914376
CCCGTAT252.8491013E-669.914373
TGCGCTA150.002231776769.914372
ATATCCC301.0266376E-769.914378
GTTCGTA207.9533456E-569.914372
Produced by FastQC (version 0.11.5)