FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_21-TCP23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_21-TCP23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946942
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG24310.12486247664285838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1900.066.275982
CGTATAG21350.066.0259255
GTATAGC21900.065.805236
GGTATAG43850.065.411115
TTATAGC25350.064.714596
GTATAGG49200.064.483636
CGGTATA26050.064.049834
CCTATAC12400.063.7520375
GCTATAG26150.063.671135
TATAGCC16550.063.617387
TATAGCG22800.063.3610657
GGCCCTA1000.062.962192
TATACGG24750.062.7501957
CGTATAC15850.062.0132265
CTATAGC17650.061.4361886
CCTATAA17500.061.363155
ATACTGG9200.061.2132388
GATATAG30350.061.198835
GGTATAA37100.061.1896675
TATAGGT53400.061.114987
Produced by FastQC (version 0.11.5)